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iscript reverse transcriptase cdna synthesis kit  (Bio-Rad)


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    Structured Review

    Bio-Rad iscript reverse transcriptase cdna synthesis kit
    Iscript Reverse Transcriptase Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 49594 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+cdna+synthesis+kit/iScript+cDNA+Synthesis+Kit/pmc13080255-182-23-29
    Average 99 stars, based on 49594 article reviews
    iscript reverse transcriptase cdna synthesis kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Loss of AMPK potentiates inflammation by activating the inflammasome after traumatic brain injury in mice
    Article Snippet: .. After stimulation with LI, WT, and AMPK-KO glia were isolated at 6 h, and total RNA was extracted using TRIzol and a RNeasy Kit (Qiagen) according to the manufacturer’s protocol. cDNA was synthesized from 1 μg of total RNA using an iScript reverse transcriptase cDNA synthesis kit (Bio-Rad) to amplify all genes. .. The primer sets and their nucleotide sequences were selected from the available online database and purchased from Integrated DNA Technology (IDT Coralville, Iowa, USA) or real-time primers.

    Synthesized:

    Article Title: Loss of AMPK potentiates inflammation by activating the inflammasome after traumatic brain injury in mice
    Article Snippet: .. After stimulation with LI, WT, and AMPK-KO glia were isolated at 6 h, and total RNA was extracted using TRIzol and a RNeasy Kit (Qiagen) according to the manufacturer’s protocol. cDNA was synthesized from 1 μg of total RNA using an iScript reverse transcriptase cDNA synthesis kit (Bio-Rad) to amplify all genes. .. The primer sets and their nucleotide sequences were selected from the available online database and purchased from Integrated DNA Technology (IDT Coralville, Iowa, USA) or real-time primers.

    Reverse Transcription:

    Article Title: Loss of AMPK potentiates inflammation by activating the inflammasome after traumatic brain injury in mice
    Article Snippet: .. After stimulation with LI, WT, and AMPK-KO glia were isolated at 6 h, and total RNA was extracted using TRIzol and a RNeasy Kit (Qiagen) according to the manufacturer’s protocol. cDNA was synthesized from 1 μg of total RNA using an iScript reverse transcriptase cDNA synthesis kit (Bio-Rad) to amplify all genes. .. The primer sets and their nucleotide sequences were selected from the available online database and purchased from Integrated DNA Technology (IDT Coralville, Iowa, USA) or real-time primers.

    Article Title: The StkSR Two-Component System Influences Colistin Resistance in Acinetobacter baumannii
    Article Snippet: Phases were separated by centrifugation and RNA extracted using the RNA Mini Kit II (Bioline, Australia) following the manufacturer’s recommendations before DNaseI treatment. .. The iScript reverse-transcriptase cDNA synthesis kit (Bio-Rad, Sydney, NSW, Australia) was used to synthesize cDNA following the manufacturer’s recommendations. .. Quantitative reverse transcription PCR was performed using iTaq TM Universal SYBR ® green supermix (Bio-Rad, Sydney, NSW, Australia) in conjunction with a Rotor-Gene Q (Qiagen, Melbourne, VIC, Australia) with oligonucleotides designed in PRIMER Biosoft NetPrimer ( www.premiersoft.com , accessed on 10 July 2016) ( ).

    Article Title: Effect of Dietary Benzoic Acid and Oregano Essential Oil as a Substitute for an Anti-Coccidial Agent on Growth Performance and Physiological and Immunological Responses in Broiler Chickens Challenged with Eimeria Species
    Article Snippet: Total RNA was treated with RNase-free DNAseI (Fermentas Life Sciences, Burlington, ON, Canada) to exclude genomic DNA contamination. .. Then, iScript Reverse Transcriptase cDNA synthesis kit (Bio-Rad Laboratories Inc., Hercules, CA, USA) was used for synthesizing cDNA. .. Quantification of mRNA level was achieved via quantitative real-time polymerase chain reaction (qPCR) (CFX96, Bio-Rad Laboratories Inc., Hercules, CA, USA) using the primers specific to IL-10 , IFN-γ , and TLR-4 genes along with the housekeeping gene ( GAPDH ) ( ) [ ].

    Article Title: Effects of Saccharomyces cerevisiae fermentation product (SCFP) and phytogenic feed additive as alternatives to antibiotic growth promoters on pathogen mitigation, immunomodulation and production performance in commercial broiler chickens
    Article Snippet: .. Constant amount (1 μg) of RNA was reverse transcribed using 4 μl of iScript Reverse Transcriptase cDNA synthesis kit (BioRad, USA) as per the manufacturer’s instruction. ..

    Article Title: Characterization of interferon gamma gene in relation to immunological responses in Haemonchus contortus resistant and susceptible Garole sheep.
    Article Snippet: Garole sheep exhibits within-breed difference in resistance to natural gastrointestinal nematode infection predominated by Haemonchus contortus.. In the present study, interferon gamma gene (IFN-γ) was characterized in relation to parasitological, haematological, and immune response against H. contortus in resistant and susceptible Garole sheep.. Resistant and susceptible Garole sheep were selected from the field based on consistent low faecal egg counts (FEC) for one year and single nucleotide polymorphisms (SNPs) in the IFN-γ gene.

    Article Title: Comparison of Th‐2 Cytokines and Associated Gene Expression in Peripheral Blood of Resistant and Susceptible Garole Sheep Infected With Haemonchus contortus
    Article Snippet: .. Reverse transcription reaction was carried out to synthesize cDNA from the extracted whole RNA samples placed in 20 μ l reaction mixtures using iScript Reverse Transcriptase cDNA synthesis kit (BioRad, United States) as per the manufacturer′s instruction. ..

    Article Title: The paracrine isthmin1 transcriptionally regulated by C/EBPβ exacerbates pulmonary vascular leakage in murine sepsis.
    Article Snippet: It is known that pulmonary vascular leakage, a key pathological feature of sepsis-induced lung injury, is largely regulated by perivascular cells.. However, the underlying mechanisms have not been fully uncovered.. In the present study, we aimed to evaluate the role of isthmin1, a secretory protein originating from alveolar epithelium, in the pulmonary vascular leakage during sepsis and to investigate the regulatory mechanisms of isthmin1 gene transcription.

    Article Title: Effects of Saccharomyces cerevisiae fermentation product (SCFP) and phytogenic feed additive as alternatives to antibiotic growth promoters on pathogen mitigation, immunomodulation and production performance in commercial broiler chickens.
    Article Snippet: .. Constant amount (1 μg) of RNA was reverse transcribed using 4 μl of iScript Reverse Transcriptase cDNA synthesis kit (BioRad, USA) as per the manufacturer’s instruction. ..

    cDNA Synthesis:

    Article Title: Loss of AMPK potentiates inflammation by activating the inflammasome after traumatic brain injury in mice
    Article Snippet: .. After stimulation with LI, WT, and AMPK-KO glia were isolated at 6 h, and total RNA was extracted using TRIzol and a RNeasy Kit (Qiagen) according to the manufacturer’s protocol. cDNA was synthesized from 1 μg of total RNA using an iScript reverse transcriptase cDNA synthesis kit (Bio-Rad) to amplify all genes. .. The primer sets and their nucleotide sequences were selected from the available online database and purchased from Integrated DNA Technology (IDT Coralville, Iowa, USA) or real-time primers.

    Article Title: The StkSR Two-Component System Influences Colistin Resistance in Acinetobacter baumannii
    Article Snippet: Phases were separated by centrifugation and RNA extracted using the RNA Mini Kit II (Bioline, Australia) following the manufacturer’s recommendations before DNaseI treatment. .. The iScript reverse-transcriptase cDNA synthesis kit (Bio-Rad, Sydney, NSW, Australia) was used to synthesize cDNA following the manufacturer’s recommendations. .. Quantitative reverse transcription PCR was performed using iTaq TM Universal SYBR ® green supermix (Bio-Rad, Sydney, NSW, Australia) in conjunction with a Rotor-Gene Q (Qiagen, Melbourne, VIC, Australia) with oligonucleotides designed in PRIMER Biosoft NetPrimer ( www.premiersoft.com , accessed on 10 July 2016) ( ).

    Article Title: Effect of Dietary Benzoic Acid and Oregano Essential Oil as a Substitute for an Anti-Coccidial Agent on Growth Performance and Physiological and Immunological Responses in Broiler Chickens Challenged with Eimeria Species
    Article Snippet: Total RNA was treated with RNase-free DNAseI (Fermentas Life Sciences, Burlington, ON, Canada) to exclude genomic DNA contamination. .. Then, iScript Reverse Transcriptase cDNA synthesis kit (Bio-Rad Laboratories Inc., Hercules, CA, USA) was used for synthesizing cDNA. .. Quantification of mRNA level was achieved via quantitative real-time polymerase chain reaction (qPCR) (CFX96, Bio-Rad Laboratories Inc., Hercules, CA, USA) using the primers specific to IL-10 , IFN-γ , and TLR-4 genes along with the housekeeping gene ( GAPDH ) ( ) [ ].

    Article Title: Effects of Saccharomyces cerevisiae fermentation product (SCFP) and phytogenic feed additive as alternatives to antibiotic growth promoters on pathogen mitigation, immunomodulation and production performance in commercial broiler chickens
    Article Snippet: .. Constant amount (1 μg) of RNA was reverse transcribed using 4 μl of iScript Reverse Transcriptase cDNA synthesis kit (BioRad, USA) as per the manufacturer’s instruction. ..

    Article Title: Characterization of interferon gamma gene in relation to immunological responses in Haemonchus contortus resistant and susceptible Garole sheep.
    Article Snippet: Garole sheep exhibits within-breed difference in resistance to natural gastrointestinal nematode infection predominated by Haemonchus contortus.. In the present study, interferon gamma gene (IFN-γ) was characterized in relation to parasitological, haematological, and immune response against H. contortus in resistant and susceptible Garole sheep.. Resistant and susceptible Garole sheep were selected from the field based on consistent low faecal egg counts (FEC) for one year and single nucleotide polymorphisms (SNPs) in the IFN-γ gene.

    Article Title: Comparison of Th‐2 Cytokines and Associated Gene Expression in Peripheral Blood of Resistant and Susceptible Garole Sheep Infected With Haemonchus contortus
    Article Snippet: .. Reverse transcription reaction was carried out to synthesize cDNA from the extracted whole RNA samples placed in 20 μ l reaction mixtures using iScript Reverse Transcriptase cDNA synthesis kit (BioRad, United States) as per the manufacturer′s instruction. ..

    Article Title: The paracrine isthmin1 transcriptionally regulated by C/EBPβ exacerbates pulmonary vascular leakage in murine sepsis.
    Article Snippet: It is known that pulmonary vascular leakage, a key pathological feature of sepsis-induced lung injury, is largely regulated by perivascular cells.. However, the underlying mechanisms have not been fully uncovered.. In the present study, we aimed to evaluate the role of isthmin1, a secretory protein originating from alveolar epithelium, in the pulmonary vascular leakage during sepsis and to investigate the regulatory mechanisms of isthmin1 gene transcription.

    Article Title: Effects of Saccharomyces cerevisiae fermentation product (SCFP) and phytogenic feed additive as alternatives to antibiotic growth promoters on pathogen mitigation, immunomodulation and production performance in commercial broiler chickens.
    Article Snippet: .. Constant amount (1 μg) of RNA was reverse transcribed using 4 μl of iScript Reverse Transcriptase cDNA synthesis kit (BioRad, USA) as per the manufacturer’s instruction. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Characterization of interferon gamma gene in relation to immunological responses in Haemonchus contortus resistant and susceptible Garole sheep.
    Article Snippet: Garole sheep exhibits within-breed difference in resistance to natural gastrointestinal nematode infection predominated by Haemonchus contortus.. In the present study, interferon gamma gene (IFN-γ) was characterized in relation to parasitological, haematological, and immune response against H. contortus in resistant and susceptible Garole sheep.. Resistant and susceptible Garole sheep were selected from the field based on consistent low faecal egg counts (FEC) for one year and single nucleotide polymorphisms (SNPs) in the IFN-γ gene.

    Expressing:

    Article Title: Characterization of interferon gamma gene in relation to immunological responses in Haemonchus contortus resistant and susceptible Garole sheep.
    Article Snippet: Garole sheep exhibits within-breed difference in resistance to natural gastrointestinal nematode infection predominated by Haemonchus contortus.. In the present study, interferon gamma gene (IFN-γ) was characterized in relation to parasitological, haematological, and immune response against H. contortus in resistant and susceptible Garole sheep.. Resistant and susceptible Garole sheep were selected from the field based on consistent low faecal egg counts (FEC) for one year and single nucleotide polymorphisms (SNPs) in the IFN-γ gene.



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